Due to his status as the last Shah of Iran, he is often known as simply the Shah. Although we show data from human samples on our webpage for this antibody, we unfortunately do not have any images from Drosophila samples that I can share with you at the present time.Mohammad Reza Pahlavi ( Persian: محمدرضا پهلوی, pronounced 26 October 1919 – 27 July 1980), also known as Mohammad Reza Shah ( محمدرضا شاه), was the last Shah ( King) of the Imperial State of Iran from 16 September 1941 until his overthrow in the Iranian Revolution on 11 February 1979. The immunogen used to generate this antibody was a synthetic peptide made to an N-terminal portion (within residues 1-100) of the drosophila SINA protein (Swiss-Prot# P21461). For further information on our guarantee please see the following link: Quality Assurance.
Our SIAH2 antibody with catalogue number NB110-88113 is covered by our 100% guarantee for the detection of SIAH2 in samples from Drosophila by Western blotting, Flow Cytometry, Immunofluorescence, and Immunohistochemistry with both frozen and paraffin-embedded samples. As in the webpage it says that this antibody recognizes Drosophila Sina we would like to ask if this antibody was tested in Drosophila tissues or Drosophila Cells. We want to try to look at Siah (sina in Drosophila) in drosophila larval brains, more specifically in the Drosophila neural stem cells. Hi, we are trying to find out if the antibody NB110-88113 (SIAH2 Antibody (24E6H3) 0.5ml Cancer Marker, Stem Cell Marker) will be useful for our application. Inhibition of RAS-mediated transformation and tumorigenesis by targeting the downstream E3 ubiquitin ligase seven in absentia homologue. Effect of disrupting seven-in-absentia homolog 2 function on lung cancer cell growth. The expression of the ubiquitin ligase SIAH2 (seven in absentia homolog 2) is mediated through gene copy number in breast cancer and is associated with a basal-like phenotype and p53 expression Breast Cancer Res 2011 Feb (IHC, Human)Īhmed, AU et al. The e3 ubiquitin ligase siah2 contributes to castration-resistant prostate cancer by regulation of androgen receptor transcriptional activity Cancer Cell 2013 Mar 18 (IHC, Human)Ĭhan P, Moller A, Liu MC et al. Evaluation of protein biomarkers of prostate cancer aggressiveness. Rizzardi AE, Rosener NK, Koopmeiners JS et al. SIAH2 antibody (clone 24E6H3) used for IHC-P on TMAs of invasive breast carcinoma and analysable cases of normal breast tissue - heat induced antigen retrieval with 10 mM sodium citrate buffer pH 6.0 for 20 min at 95C, blocking with 5% goat serum for 1 hour, endogenous peroxidase activity blocked with30 min incubation in 0.3% H2O2, primary used at 1:40 dilution, detection via HRP-conjugated secondary and DAB Substrate Kit from MaiXin Bio (Figure 4i). Hypoxia regulates Hippo signalling through the SIAH2 ubiquitin E3 ligase. The ubiquitin ligase Siah is a novel regulator of Zeb1 in breast cancer. 2014 Dec 27 (IHC-P, Human)Ĭhen Anna, Wong Christina S F, Liu Mira C P et al. Increased expression of PHD3 represses the HIF-1 signaling pathway and contributes to poor neovascularization in pancreatic ductal adenocarcinoma. Combining clinicopathological predictors and molecular biomarkers in the oncogenic K-RAS/Ki67/HIF-1alpha pathway to predict survival in resectable pancreatic cancer Br. 
SIAH2 antibody (clone 24E6H3, NB110-88113) was used for WB on lysates of endothelial cells isolated from lungs of wildtype (WT) and Siah2?/? (KO) mice. Loss of Siah2 does not impact angiogenic potential of murine endothelial cells.
The Expression of the Ubiquitin Ligase SIAH2 (Seven In Absentia Homolog 2) Is Increased in Human Lung Cancer. Moreno P, Lara-Chica M, Soler-Torronteras R et al. Zyxin-Siah2-Lats2 axis mediates cooperation between Hippo and TGF-beta signalling pathways. SIAH2 protein expression in breast cancer is inversely related with ER status and outcome to tamoxifen therapy. SIAH and EGFR, Two RAS Pathway Biomarkers, are Highly Prognostic in Locally Advanced and Metastatic Breast Cancer. Van Reesema LL, Zheleva V, Winston JS et al. DHX15 promotes prostate cancer progression by stimulating Siah2-mediated ubiquitination of androgen receptor. The SIAH2-NRF1 axis spatially regulates tumor microenvironment remodeling for tumor progression Nat Commun (IHC, Human) Hughes SE, Hemenway E, Guo F The E3 ubiquitin ligase Sina regulates the assembly and disassembly of the synaptonemal complex in Drosophila females PLoS Genet.
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